蒙古马运动相关基因的表达量差异研究

doi:10.11843/j.issn.0366-6964.2013.12.006

Abstract

Abstract:

To understand the genes related to endurance of Mongolia horses, the differential expression of genes related to exercise before and after exercise was detected in this study. The expression changes of 7 genes related with energy metabolism and muscle growth were measured in Mongolian horses skeletal muscle at three stages (pre-exercise (T₀), immediately post 10 000 m endurance exercise (T₁) and 4 h post exercise (T₂)) by using quantitative RT-PCR. And protein expression of the genes which mRNA expression levels were significantly different at the three stages were observed using Western blotting. The results showed that mRNA expression level of two genes（PPARGC1A and PDK4）increased significantly at T₂ stage than that at T₀ stage. Western blotting results also showed the protein expression of two genes after endurance sports had increased. These results reveal that the PPARGC1A and PDK4 are closely related to exercise. The above results provide some data for further study of the Mongolia horse exercise.

CLC Number:

S821.2

YANG Li-hua，BAI Dong-yi，Gaoyahan，Wrguml，Srguge，Manglai. Characterization of Exercise Relevant Genes in Mongolian Horses[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1900-1906.

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Characterization of Exercise Relevant Genes in Mongolian Horses

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